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dc.contributor.authorZhou, Y.
dc.contributor.authorYang, Y.
dc.contributor.authorGuo, L.
dc.contributor.authorQian, J.
dc.contributor.authorGe, J.
dc.contributor.authorSinner, D.
dc.contributor.authorDing, H.
dc.contributor.authorCalifano, A.
dc.contributor.authorCardoso, W.V.
dc.date.accessioned2022-12-15T22:41:34Z
dc.date.available2022-12-15T22:41:34Z
dc.date.issued2022
dc.identifier.citationZhou, Y., Yang, Y., Guo, L., Qian, J., Ge, J., Sinner, D., Ding, H., Califano, A., & Cardoso, W. V. (2022). Airway basal cells show regionally distinct potential to undergo metaplastic differentiation. ELife, 11.
dc.identifier.issn2050-084X
dc.identifier.pmid36178196
dc.identifier.doi10.7554/eLife.80083
dc.identifier.urihttp://hdl.handle.net/10150/667232
dc.description.abstractBasal cells are multipotent stem cells of a variety of organs, including the respiratory tract, where they are major components of the airway epithelium. However, it remains unclear how diverse basal cells are and how distinct subpopulations respond to airway challenges. Using single cell RNA-sequencing and functional approaches, we report a significant and previously underappreciated degree of heterogeneity in the basal cell pool, leading to identification of six subpopulations in the adult murine trachea. Among these, we found two major subpopulations, collectively comprising the most uncommitted of all the pools, but with distinct gene expression signatures. Notably, these occupy distinct ventral and dorsal tracheal niches and differ in their ability to self-renew and initiate a program of differentiation in response to environmental perturbations in primary cultures and in mouse injury models in vivo. We found that such heterogeneity is acquired prenatally, when the basal cell pool and local niches are still being established, and depends on the integrity of these niches, as supported by the altered basal cell phenotype of tracheal cartilage-deficient mouse mutants. Finally, we show that features that distinguish these progenitor subpopulations in murine airways are conserved in humans. Together, the data provide novel insights into the origin and impact of basal cell heterogeneity on the establishment of regionally distinct responses of the airway epithelium during injury-repair and in disease conditions. © 2022, Zhou, Yang, Guo et al.
dc.language.isoen
dc.publishereLife Sciences Publications
dc.rightsCopyright © Zhou, Yang, Guo et al. This article is distributed under the terms of the Creative Commons Attribution License.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectairway epithelium
dc.subjectbasal cell heterogeneity
dc.subjectdevelopmental biology
dc.subjecthuman
dc.subjectlung repair
dc.subjectmouse
dc.subjectorganotypic cultures
dc.subjectregenerative medicine
dc.subjectstem cell
dc.subjectstem cells
dc.titleAirway basal cells show regionally distinct potential to undergo metaplastic differentiation
dc.typeArticle
dc.typetext
dc.contributor.departmentDepartment of Pharmacy Practice and Science, College of Pharmacy, University of Arizona
dc.identifier.journaleLife
dc.description.noteOpen access journal
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
dc.eprint.versionFinal published version
dc.source.journaltitleeLife
refterms.dateFOA2022-12-15T22:41:34Z


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Copyright © Zhou, Yang, Guo et al. This article is distributed under the terms of the Creative Commons Attribution License.
Except where otherwise noted, this item's license is described as Copyright © Zhou, Yang, Guo et al. This article is distributed under the terms of the Creative Commons Attribution License.