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dc.contributor.advisorTomasiak , Thomas
dc.contributor.authorAguilar, Briana
dc.creatorAguilar, Briana
dc.date.accessioned2023-08-17T04:31:15Z
dc.date.available2023-08-17T04:31:15Z
dc.date.issued2023
dc.identifier.citationAguilar, Briana. (2023). GENERATION OF A CYSTEINE-LESS BACKBONE YCF1 PROTEIN FOR ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY (Bachelor's thesis, University of Arizona, Tucson, USA).
dc.identifier.urihttp://hdl.handle.net/10150/668534
dc.description.abstractThere are a lot of research applications to YCF1 including research treatments for various diseases. One of those illnesses, cystic fibrosis, has a protein that does not work well known as the cystic fibrosis transmembrane conductance regulator, CFTR. YCF1 and CFTR are homologs, thus the research conducted on understanding cysteine modification and conformation space could be utilized for understanding how to improve current treatment options for cystic fibrosis. The goal of the research is to have a multiple cysteine-less mutated YCF1’s that are purified and ready for their confirmational space to be examined. The following was utilized for purification: primer design for mutagenesis, Q5 mini-prep, QIAprep spin miniprep kit, yeast growth, and nickel purification. The primary technique being used to examine the conformational space is EPR, and Cryo-EM could further confirm details about the orientation. The end result of the lab was purified proteins that were thoroughly purified and ready for EPR spin labels to be attached.
dc.language.isoen
dc.publisherThe University of Arizona.
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subject
dc.titleGENERATION OF A CYSTEINE-LESS BACKBONE YCF1 PROTEIN FOR ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY
dc.typeElectronic thesis
dc.typetext
thesis.degree.grantorUniversity of Arizona
thesis.degree.levelbachelors
thesis.degree.disciplineBiochemistry
thesis.degree.disciplineHonors College
thesis.degree.nameB.S.
refterms.dateFOA2023-08-17T04:31:15Z


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