EFFECTS OF KALIKREIN RELATED PEPTIDASE 6 (KLK6) OVEREXPRESSION IN COLORECTAL CANCER CELLS GROWTH AND VIABILITY
AuthorArana Garrido, Rodrigo
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PublisherThe University of Arizona.
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AbstractColorectal cancer (CRC) is one of the most common and more life-threatening cancers. Its prognosis improves greatly with early detection, which is why its important to develop biomarkers that can be used to detect the disease in its early stages. KLK6 is a secreted protein commonly overexpressed in CRC due to its effects on cell growth, apoptosis and metastasis. Here we created KLK6 knockouts and heterozygous clones from the SW480 cell line and measured cell growth and viability over multiple days. The knockouts were successfully created and the effectiveness of the CRIPSR silencing was proven by real time PCR to measure mRNA expression, and ELISA to measure KLK6 protein secretion. The heterozygous clones however, showed either normal expression of KLK6, or complete silencing of the gene, verified by the same methods mentioned. The clones which did not express KLK6 showed much slower growth and viability compared to the cell lines which normally expressed the protein.