Handheld cross-polarised microscope for imaging individual pigmented cells in human skin in vivo
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Author
Romero, RafaelZhao, Jingwei
Stratton, Delaney
Marcelino, Kenneth
Sugimura, Momoka
Nichols, Alia
Gonzalez, Salvador
Jain, Manu
Curiel-Lewandrowski, Clara
Kang, Dongkyun
Affiliation
Department of Biomedical Engineering, University of ArizonaCollege of Optical Sciences, University of Arizona
Division of Dermatology, College of Medicine, University of Arizona
Issue Date
2023-09-17Keywords
capillary imagingcross-polarised microscopy
in vivo microscopy
melanin imaging
Skin Imaging
three-dimensional microscopy
Metadata
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John Wiley and Sons IncCitation
Romero, R., Zhao, J., Stratton, D., Marcelino, K., Sugimura, M., Nichols, A., ... & Kang, D. (2023). Handheld cross‐polarized microscope for imaging individual pigmented cells in human skin in vivo. Journal of Microscopy.Journal
Journal of microscopyRights
© 2023 Royal Microscopical Society.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
We present the development of a simple, handheld cross-polarised microscope (CPM) and demonstration of imaging individual pigmented cells in human skin in vivo. In the CPM device, the cross-polarised detection approach is used to reduce the specular reflection from the skin surface and preferentially detect multiply-scattered light. The multiply-scattered light works as back illumination from within the tissue towards the skin surface, and superficial pigment such as intraepidermal melanin absorbs some spectral bands of the multiply-scattered light and cast coloured shadows. Since the light that interacted with the superficial pigment only needs to travel a short distance before it exits the skin surface, microscopic details of the pigment can be preserved. The CPM device uses a water-immersion objective lens with a high numerical aperture to image the microscopic details with minimal spherical aberrations and a small depth of focus. Preliminary results from a pilot study of imaging skin lesions in vivo showed that the CPM device could reveal three-dimensional distribution of pigmented cells and intracellular distribution of pigment. Co-registered CPM and reflectance confocal microscopy images showed good correspondence between dark, brown cells in CPM images and bright, melanin-containing cells in reflectance confocal microscopy images.Note
12 month embargo; first published: 17 September 2023EISSN
1365-2818PubMed ID
37698068Version
Final accepted manuscriptae974a485f413a2113503eed53cd6c53
10.1111/jmi.13225
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