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    Handheld cross-polarised microscope for imaging individual pigmented cells in human skin in vivo

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    Author
    Romero, Rafael
    Zhao, Jingwei
    Stratton, Delaney
    Marcelino, Kenneth
    Sugimura, Momoka
    Nichols, Alia
    Gonzalez, Salvador
    Jain, Manu
    Curiel-Lewandrowski, Clara
    Kang, Dongkyun
    Affiliation
    Department of Biomedical Engineering, University of Arizona
    College of Optical Sciences, University of Arizona
    Division of Dermatology, College of Medicine, University of Arizona
    Issue Date
    2023-09-17
    Keywords
    capillary imaging
    cross-polarised microscopy
    in vivo microscopy
    melanin imaging
    Skin Imaging
    three-dimensional microscopy
    
    Metadata
    Show full item record
    Publisher
    John Wiley and Sons Inc
    Citation
    Romero, R., Zhao, J., Stratton, D., Marcelino, K., Sugimura, M., Nichols, A., ... & Kang, D. (2023). Handheld cross‐polarized microscope for imaging individual pigmented cells in human skin in vivo. Journal of Microscopy.
    Journal
    Journal of microscopy
    Rights
    © 2023 Royal Microscopical Society.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    We present the development of a simple, handheld cross-polarised microscope (CPM) and demonstration of imaging individual pigmented cells in human skin in vivo. In the CPM device, the cross-polarised detection approach is used to reduce the specular reflection from the skin surface and preferentially detect multiply-scattered light. The multiply-scattered light works as back illumination from within the tissue towards the skin surface, and superficial pigment such as intraepidermal melanin absorbs some spectral bands of the multiply-scattered light and cast coloured shadows. Since the light that interacted with the superficial pigment only needs to travel a short distance before it exits the skin surface, microscopic details of the pigment can be preserved. The CPM device uses a water-immersion objective lens with a high numerical aperture to image the microscopic details with minimal spherical aberrations and a small depth of focus. Preliminary results from a pilot study of imaging skin lesions in vivo showed that the CPM device could reveal three-dimensional distribution of pigmented cells and intracellular distribution of pigment. Co-registered CPM and reflectance confocal microscopy images showed good correspondence between dark, brown cells in CPM images and bright, melanin-containing cells in reflectance confocal microscopy images.
    Note
    12 month embargo; first published: 17 September 2023
    EISSN
    1365-2818
    PubMed ID
    37698068
    DOI
    10.1111/jmi.13225
    Version
    Final accepted manuscript
    ae974a485f413a2113503eed53cd6c53
    10.1111/jmi.13225
    Scopus Count
    Collections
    UA Faculty Publications

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