Show simple item record

dc.contributor.authorSoria-Bustos, J.
dc.contributor.authorSaldaña-Ahuactzi, Z.
dc.contributor.authorSamadder, P.
dc.contributor.authorYañez-Santos, J.A.
dc.contributor.authorLaguna, Y.M.
dc.contributor.authorCedillo-Ramírez, M.L.
dc.contributor.authorGirón, J.A.
dc.date.accessioned2023-12-21T18:45:40Z
dc.date.available2023-12-21T18:45:40Z
dc.date.issued2022-11-08
dc.identifier.citationSoria-Bustos, J.; Saldaña- Ahuactzi, Z.; Samadder, P.; Yañez- Santos, J.A.; Laguna, Y.M.; Cedillo-Ramírez, M.L.; Girón, J.A. The Assembly of Flagella in Enteropathogenic Escherichia coli Requires the Presence of a Functional Type III Secretion System. Int. J. Mol. Sci. 2022, 23, 13705.
dc.identifier.issn1661-6596
dc.identifier.doi10.3390/ijms232213705
dc.identifier.urihttp://hdl.handle.net/10150/670405
dc.description.abstractIn enteropathogenic Escherichia coli (EPEC), the production of flagella and the type III secretion system (T3SS) is activated in the presence of host cultured epithelial cells. The goal of this study was to investigate the relationship between expression of flagella and the T3SS. Mutants deficient in assembling T3SS basal and translocon components (ΔespA, ΔespB, ΔespD, ΔescC, ΔescN, and ΔescV), and in secreting effector molecules (ΔsepD and ΔsepL) were tested for flagella production under several growth conditions. The ΔespA mutant did not produce flagella in any condition tested, although fliC was transcribed. The remaining mutants produced different levels of flagella upon growth in LB or in the presence of cells but were significantly diminished in flagella production after growth in Dulbecco’s minimal essential medium. We also investigated the role of virulence and global regulator genes in expression of flagella. The ΔqseB and ΔqseC mutants produced abundant flagella only when growing in LB and in the presence of HeLa cells, indicating that QseB and QseC act as negative regulators of fliC transcription. The ΔgrlR, ΔperA, Δler, Δhns, and Δfis mutants produced low levels of flagella, suggesting these regulators are activators of fliC expression. These data suggest that the presence of an intact T3SS is required for assembly of flagella highlighting the existence in EPEC of a cross-talk between these two virulence-associated T3SSs. © 2022 by the authors.
dc.language.isoen
dc.publisherMDPI
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectenteropathogenic
dc.subjectEscherichia coli
dc.subjectflagella
dc.subjectT3SS
dc.subjectvirulence
dc.titleThe Assembly of Flagella in Enteropathogenic Escherichia coli Requires the Presence of a Functional Type III Secretion System
dc.typeArticle
dc.typetext
dc.contributor.departmentDepartment of Immunobiology, University of Arizona
dc.identifier.journalInternational Journal of Molecular Sciences
dc.description.noteOpen access journal
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
dc.eprint.versionFinal published version
dc.source.journaltitleInternational Journal of Molecular Sciences
refterms.dateFOA2023-12-21T18:45:40Z


Files in this item

Thumbnail
Name:
ijms-23-13705-v2.pdf
Size:
2.901Mb
Format:
PDF
Description:
Final Published Version

This item appears in the following Collection(s)

Show simple item record

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).