We are upgrading the repository! A content freeze is in effect until November 22nd, 2024 - no new submissions will be accepted; however, all content already published will remain publicly available. Please reach out to repository@u.library.arizona.edu with your questions, or if you are a UA affiliate who needs to make content available soon. Note that any new user accounts created after September 22, 2024 will need to be recreated by the user in November after our migration is completed.
Transcriptomic analysis of diabetic kidney disease and neuropathy in mouse models of type 1 and type 2 diabetes
Author
Elzinga, S.E.Eid, S.A.
McGregor, B.A.
Jang, D.-G.
Hinder, L.M.
Dauch, J.R.
Hayes, J.M.
Zhang, H.
Guo, K.
Pennathur, S.
Kretzler, M.
Brosius, F.C.
Koubek, E.J.
Feldman, E.L.
Hur, J.
Affiliation
Department of Medicine, University of ArizonaIssue Date
2023-10-04Keywords
DiabetesDiabetic kidney disease
Diabetic peripheral neuropathy
Genetic mouse models
RNA-seq
Self-organizing map
Metadata
Show full item recordPublisher
Company of Biologists LtdCitation
Sarah E. Elzinga, Stephanie A. Eid, Brett A. McGregor, Dae-Gyu Jang, Lucy M. Hinder, Jacqueline R. Dauch, John M. Hayes, Hongyu Zhang, Kai Guo, Subramaniam Pennathur, Matthias Kretzler, Frank C. Brosius, Emily J. Koubek, Eva L. Feldman, Junguk Hur; Transcriptomic analysis of diabetic kidney disease and neuropathy in mouse models of type 1 and type 2 diabetes. Dis Model Mech 1 October 2023; 16 (10): dmm050080. doi: https://doi.org/10.1242/dmm.050080Rights
© 2023. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Diabetic kidney disease (DKD) and diabetic peripheral neuropathy (DPN) are common complications of type 1 (T1D) and type 2 (T2D) diabetes. However, the mechanisms underlying pathogenesis of these complications are unclear. In this study, we optimized a streptozotocin-induced db/+ murine model of T1D and compared it to our established db/db T2D mouse model of the same C57BLKS/J background. Glomeruli and sciatic nerve transcriptomic data from T1D and T2D mice were analyzed by self-organizing map and differential gene expression analysis. Consistent with prior literature, pathways related to immune function and inflammation were dysregulated in both complications in T1D and T2D mice. Gene-level analysis identified a high degree of concordance in shared differentially expressed genes (DEGs) in both complications and across diabetes type when using mice from the same cohort and genetic background. As we have previously shown a low concordance of shared DEGs in DPN when using mice from different cohorts and genetic backgrounds, this suggests that genetic background may influence diabetic complications. Collectively, these findings support the role of inflammation and indicate that genetic background is important in complications of both T1D and T2D. © 2023 Company of Biologists Ltd. All rights reserved.Note
Open access journalISSN
1754-8403PubMed ID
37791586Version
Final Published Versionae974a485f413a2113503eed53cd6c53
10.1242/dmm.050080
Scopus Count
Collections
Except where otherwise noted, this item's license is described as © 2023. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Related articles
- Transcriptional networks of murine diabetic peripheral neuropathy and nephropathy: common and distinct gene expression patterns.
- Authors: Hur J, O'Brien PD, Nair V, Hinder LM, McGregor BA, Jagadish HV, Kretzler M, Brosius FC 3rd, Feldman EL
- Issue date: 2016 Jun
- Conserved Transcriptional Signatures in Human and Murine Diabetic Peripheral Neuropathy.
- Authors: McGregor BA, Eid S, Rumora AE, Murdock B, Guo K, de Anda-Jáuregui G, Porter JE, Feldman EL, Hur J
- Issue date: 2018 Dec 5
- Gene expression profiles of diabetic kidney disease and neuropathy in eNOS knockout mice: Predictors of pathology and RAS blockade effects.
- Authors: Eid SA, Hinder LM, Zhang H, Eksi R, Nair V, Eddy S, Eichinger F, Park M, Saha J, Berthier CC, Jagadish HV, Guan Y, Pennathur S, Hur J, Kretzler M, Feldman EL, Brosius FC
- Issue date: 2021 May
- IGF2BP2 and IGF2 genetic effects in diabetes and diabetic nephropathy.
- Authors: Gu T, Horová E, Möllsten A, Seman NA, Falhammar H, Prázný M, Brismar K, Gu HF
- Issue date: 2012 Sep-Oct
- Bioinformatics prediction and experimental verification of key biomarkers for diabetic kidney disease based on transcriptome sequencing in mice.
- Authors: Zhao J, He K, Du H, Wei G, Wen Y, Wang J, Zhou X, Wang J
- Issue date: 2022