Protocol for measuring mitochondrial size in mouse and human liver tissues
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Final Published Version
Affiliation
Institute of Clinical Translational Sciences, University of ArizonaDepartments of Internal Medicine, University of Arizona College of Medicine-Phoenix
Issue Date
2024-01-18Metadata
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Cell PressCitation
Ramatchandirin, Balamurugan, et al. "Protocol for measuring mitochondrial size in mouse and human liver tissues." STAR protocols 5.1 (2024): 102842.Journal
STAR ProtocolsRights
© 2024 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Mitochondrial dynamic process is important for cell viability, metabolic activity, and mitochondria health. Here, we present a protocol for measuring mitochondrial size through immunofluorescence staining, confocal imaging, and analysis in ImageJ. We describe the steps for tissue processing, antigen retrieval, mitochondrial staining using an integrating immunofluorescence assay, and computerized image analysis to measure each mitochondrial size in mouse and human liver tissues. This protocol reduces tissue sample volume and processing time for the preparation of primary cells. For complete details on the use and execution of this protocol, please refer to Pearah et al.1 © 2024 The Author(s)Note
Open access journalISSN
2666-1667PubMed ID
38244201Version
Final Published VersionScopus Count
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Except where otherwise noted, this item's license is described as © 2024 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).