Human Cytomegalovirus Encodes Proteins involved in Altering Sterol Regulatory Element Binding Protein Activity

Abstract

Human cytomegalovirus (HCMV) is a common pathogen that causes significant complications in immunocompromised individuals while causing an asymptomatic infection in those with typical immune function. HCMV replication requires manipulation of the host's cellular machinery to produce favorable conditions for the virus. This study investigates the role of proteins encoded by HCMV and their influence in altering host lipid metabolism, specifically through the activation of sterol regulatory element binding proteins (SREBPs). Using previously annotated HCMV genes involved in altering host metabolism as a template, a dual luciferase assay was used to screen two viral genes for their potential to increase the maturation of SREBPs in vitro. This investigation furthers our understanding of HCMVs manipulation of the host lipid metabolism and its impact on infection. From our findings insight is gained towards exploring how intervening with the changes in host metabolism can influence viral replication cycles, potentially inspiring future therapeutic strategies.