Identification of the Flavone-Inducible Counter-Defense Genes and Their cis-Elements in Helicoverpa armigera
Affiliation
Department of Entomology and BIO5 Institute, University of ArizonaIssue Date
2023-05-29Keywords
AREcarboxylesterase
cis-transcriptional regulation
detoxification metabolism
flavone
Helicoverpa armigera
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Deng, Z.; Zhang, Y.; Fang, L.; Zhang, M.; Wang, L.; Ni, X.; Li, X. Identification of the Flavone-Inducible Counter-Defense Genes and Their cis-Elements in Helicoverpa armigera. Toxins 2023, 15, 365. https://doi.org/10.3390/toxins15060365Journal
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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Flavone is widely found in plants and plays an important role in plant defense against pests. Many pests, such as Helicoverpa armigera, use flavone as a cue to upregulate counter-defense genes for detoxification of flavone. Yet the spectrum of the flavone-inducible genes and their linked cis-regulatory elements remains unclear. In this study, 48 differentially expressed genes (DEGs) were found by RNA-seq. These DEGs were mainly concentrated in the retinol metabolism and drug metabolism-cytochrome P450 pathways. Further in silico analysis of the promoter regions of 24 upregulated genes predicted two motifs through MEME and five previously characterized cis-elements including CRE, TRE, EcRE, XRE-AhR and ARE. Functional analysis of the two predicted motifs and two different versions of ARE (named ARE1 and ARE2) in the promoter region of the flavone-inducible carboxylesterase gene CCE001j verified that the two motifs and ARE2 are not responsible for flavone induction of H. armigera counter-defense genes, whereas ARE1 is a new xenobiotic response element to flavone (XRE-Fla) and plays a decisive role in flavone induction of CCE001j. This study is of great significance for further understanding the antagonistic interaction between plants and herbivorous insects. © 2023 by the authors.Note
Open access journalISSN
2072-6651PubMed ID
37368666Version
Final Published Versionae974a485f413a2113503eed53cd6c53
10.3390/toxins15060365
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Except where otherwise noted, this item's license is described as © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.
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