Accelerated absorption of regular insulin administered via a vascularizing permeable microchamber implanted subcutaneously in diabetic Rattus norvegicus
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Steyn, L.V.Drew, D.
Vlachos, D.
Huey, B.
Cocchi, K.
Price, N.D.
Johnson, R.
Putnam, C.W.
Papas, K.K.
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Institute for Cellular Transplantation, Department of Surgery, University of Arizona College of MedicineIssue Date
2023-06-29
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Steyn LV, Drew D, Vlachos D, Huey B, Cocchi K, Price ND, et al. (2023) Accelerated absorption of regular insulin administered via a vascularizing permeable microchamber implanted subcutaneously in diabetic Rattus norvegicus. PLoS ONE 18(6): e0278794. https://doi.org/10.1371/journal.pone.0278794Journal
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© 2023 Steyn et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
In Type 1 diabetes patients, even ultra-rapid acting insulins injected subcutaneously reach peak concentrations in 45 minutes or longer. The lag time between dosing and peak concentration, as well as intra- and inter-subject variability, render prandial glucose control and dose consistency difficult. We postulated that insulin absorption from subcutaneously implantable vascularizing microchambers would be significantly faster than conventional subcutaneous injection. Male athymic nude R. norvegicus rendered diabetic with streptozotocin were implanted with vascularizing microchambers (single chamber; 1.5 cm2 surface area per side; nominal volume, 22.5 μl). Plasma insulin was assayed after a single dose (1.5 U/kg) of diluted insulin human (Humulin®R U-100), injected subcutaneously or via microchamber. Microchambers were also implanted in additional animals and retrieved at intervals for histologic assessment of vascularity. Following conventional subcutaneous injection, the mean peak insulin concentration was 22.7 (SD 14.2) minutes. By contrast, when identical doses of insulin were injected via subcutaneous microchamber 28 days after implantation, the mean peak insulin time was shortened to 7.50 (SD 4.52) minutes. Peak insulin concentrations were similar by either route; however, inter-subject variability was reduced when insulin was administered via microchamber. Histologic examination of tissue surrounding microchambers showed mature vascularization on days 21 and 40 post-implantation. Implantable vascularizing microchambers of similar design may prove clinically useful for insulin dosing, either intermittently by needle, or continuously by pump including in "closed loop"systems, such as the artificial pancreas. © 2023 Steyn et al.Note
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1932-6203PubMed ID
37384782Version
Final Published Versionae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0278794
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Except where otherwise noted, this item's license is described as © 2023 Steyn et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
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