Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples

Breshears, L.E.; Nguyen, B.T.; Akarapipad, P.; Sosnowski, K.; Kaarj, K.; Quirk, G.; Uhrlaub, J.L.; Nikolich-Žugich, J.; Worobey, M.; Yoon, J.-Y.

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Author

Breshears, L.E.
Nguyen, B.T.
Akarapipad, P.
Sosnowski, K.
Kaarj, K.
Quirk, G.
Uhrlaub, J.L.
Nikolich-Žugich, J.
Worobey, M.
Yoon, J.-Y.

Affiliation

Department of Biomedical Engineering, The University of Arizona
Department of Biosystems Engineering, The University of Arizona
Department of Ecology and Evolutionary Biology, The University of Arizona
Department of Immunobiology, The University of Arizona

Issue Date

2022-03-16

Citation

Lane E Breshears, Brandon T Nguyen, Patarajarin Akarapipad, Katelyn Sosnowski, Kattika Kaarj, Grace Quirk, Jennifer L Uhrlaub, Janko Nikolich-Žugich, Michael Worobey, Jeong-Yeol Yoon, Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples, PNAS Nexus, Volume 1, Issue 1, March 2022, pgac028, https://doi.org/10.1093/pnasnexus/pgac028

Journal

PNAS Nexus

Rights

© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/).

Collection Information

This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.

Abstract

Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n = 27 human subjects with n = 13 RT-qPCR positives. ©C The Author(s) 2022.

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Open access journal

ISSN

2752-6542

DOI

10.1093/pnasnexus/pgac028

Version

Final Published Version

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UA Faculty Publications

© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/).

Except where otherwise noted, this item's license is described as © The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/).